Genetic Classification and Distinguishing of Staphylococcus Species Based on Different Partial gap , 16S rRNA, hsp60 , rpoB , sodA , and tuf Gene Sequences
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چکیده
منابع مشابه
Species identification and phylogenetic relationships based on partial HSP60 gene sequences within the genus Staphylococcus.
The phylogenetic relationships among 36 validly described species or subspecies within the genus Staphylococcus were investigated by cloning and sequencing their 60 kDa heat-shock protein (HSP60) genes using a set of universal degenerate HSP60 PCR primers. The cloned partial HSP60 DNA sequences from nine Staphylococcus aureus strains were highly conserved (97-100% DNA sequence similarity; mean ...
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Seventy phytoplasma isolates, including 10 previously characterized reference strains, of the aster yellows group were examined by RFLP analysis of PCR-amplified rDNA and RFLP and sequence analysis of the tuf gene. On the basis of rDNA restriction profiles, seven previously proposed 16S rDNA subgroups (16SrI-A, -B, -C, -D, -E, -F and -K) were recognized in the material examined. In addition, th...
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Pasteurella multocida is known as an important heterogenic bacterial agent causes some severe diseases such as fowl cholera in poultry and haemorrhagic septicaemia in cattle and buffalo. A polymerase chain reaction (PCR) assay was developed using primers derived from conserved part of 16S-23S rRNA gene. The PCR amplified a fragment size of 0.7 kb using DNA from nine avian P. multocida isolates...
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15 صفحه اولPhylogenetic study and identification of human pathogenic Vibrio species based on partial hsp60 gene sequences.
The use of hsp60 gene sequences for phylogenetic study and identification of pathogenic marine vibrios was investigated. A 600-bp partial hsp60 gene was amplified by PCR and sequenced from 29 strains representing 15 Vibrio species within the family Vibrionaceae. Sequence comparison of the amplified partial hsp60 gene revealed 71-82% sequence identity among different Vibrio species and 96-100% s...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2008
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02058-07